HIGH-THROUGHPUT SCREENING OF EXPRESSED DNA LIBRARIES IN FILAMENTOUS FUNGI

摘要

The invention provides a method for the expression of exogenous DNA librarie s in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glycosylation and protein folding. The invention provides for the us e of fungi with altered morphology, which permits high-throughput screening an d directed molecular evolution of expressed proteins. The same transformed fun gi may be used (as shown in figure 1) to produce larger quantities of protein f or isolation, characterization, and application testing, and may be suitable fo r commercial production of the protein as well.