A method is described for use in whole genome analysis. The method - termed inter-population perfectly matched duplex depletion - can overcome many of the limitations of current approaches based upon SNPs and linkage disequilibrium within isolated populations. Inter-population perfectly matched duplex depletion isolates a fragment (or fragments) containing differences between the "affected" and "unaffected" populations or cells. A convenient method - terminal restriction site profiling arrays (TRSPAs) - is described for the analysis of such fragments. A totally diagnostic internal control DNA is also described which allows both the extent and exact nature of any partial digestion to be unambiguously determined for inter-population perfectly matched duplex depletion or TRSPA restriction.
申请公布号
WO0055364(A3)
申请公布日期
2001.10.11
申请号
WO2000GB00916
申请日期
2000.03.10
申请人
AMERSHAM PHARMACIA BIOTECH UK LIMITED;REEVE, MICHAEL, ALAN;WORKMAN, NICHOLAS, IAN;MARTIN-PARRAS, LUIS
发明人
REEVE, MICHAEL, ALAN;WORKMAN, NICHOLAS, IAN;MARTIN-PARRAS, LUIS