发明名称 |
Methods for sequencing repetitive sequences and for determining the order of sequence subfragments |
摘要 |
The present invention provides a method for detecting a target nucleic acid species including the steps of providing an array of probes affixed to a substrate and a plurality of labeled probes wherein each labeled probe is selected to have a first nucleic acid sequence which is complementary to a first portion of a target nucleic acid and wherein the nucleic acid sequence of at least one probe affixed to the substrate is complementary to a second portion of the nucleic acid sequence of the target, the second portion being adjacent to the first portion; applying a target nucleic acid to the array under suitable conditions for hybridization of probe sequences to complementary sequences; introducing a labeled probe to the array; hybridizing a probe affixed to the substrate to the target nucleic acid; hybridizing the labeled probe to the target nucleic acid; affixing the labeled probe to an adjacently hybridized probe in the array; and detecting the labeled probe affixed to the probe in the array.
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申请公布号 |
US6297006(B1) |
申请公布日期 |
2001.10.02 |
申请号 |
US19970812951 |
申请日期 |
1997.03.04 |
申请人 |
HYSEQ, INC. |
发明人 |
DRMANAC RADOJE T.;DRMANAC SNEZANA;HOU AARON;HAUSER BRIAN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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