| 摘要 |
PROBLEM TO BE SOLVED: To provide a method of genetic diagnosis for (method for detecting genes involved) bovine molybdenum cofactor sulfurase deficiency intended for preventing onset of the disease through screening the disease's carrier. SOLUTION: This method of genetic diagnosis for bovine molybdenum cofactor sulfurase deficiency comprises the following: in view of the fact that the above disease is attributable to deficit of a specific portion of a specific site of molybdenum cofactor sulfurase (Mcsu) gene, thus, based on the deficit, a size difference for a product with a domain containing the above deficient gene amplified by the use of a specific PCR primer is utilized; more precisely, a domain around the gene in question is specifically amplified to sense the deficit; thereby enabling bovine Mcsu deficiency and the disease's carrier to be easily and quickly detected and the disease to be diagnosed. |
