Process for the fermentative production of L-amino acids employing coryneform bacteria in which the glyA gene activity has been decreased

摘要

Production of L-amino acids (A) by fermenting an (A)-producing coryneform bacterium in which the glyA (serine hydroxymethyltransferase) gene (I) has been suppressed; enriching (A) in cells or medium, then recovering it. Independent claims are also included for the following: (1) coryneform bacteria in which (I) has been suppressed; (2) the vector pK18mobglyA' (map reproduced), deposited in Escherichia coli as DSM 13170; and (3) isolated polynucleotide (II) selected from: (a) the lacI-tac-5'-glyA unit (2000 bp sequence (S2), defined in the specification) or the lacI-tac-glyA unit (2866 bp sequence (3), defined in the specification); (b) an equivalent of (a) within the degeneracy of the genetic code; (c) a polynucleotide that hybridizes to the complement of (a) or (b); or (d) a polynucleotide that is a functionally neutral sense mutant of (S2) or (S3).