发明名称 |
Methods for detecting and enumerating campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains |
摘要 |
The present invention provides a process for detecting and enumerating <i>Campylobacter jejuni</i> in an environmental sample. The present invention further provides a process which can distinguish antibiotic resistant strains of <i>Campylobacter jejuni</i> from wild-type strains, in particular, antibiotic-resistant strains resistant to high levels of ciprofloxacin. Both processes use PCR primers which flank a target sequence unique to <i>Campylobacter jejuni</i> in combination with one or more dual-labeled oligonucleotide probes complementary to the target sequence wherein the dual-labeled probes enable detection of PCR amplification by fluorescence detection means. |
申请公布号 |
AU7348500(A) |
申请公布日期 |
2001.04.10 |
申请号 |
AU20000073485 |
申请日期 |
2000.09.05 |
申请人 |
MICHIGAN STATE UNIVERSITY |
发明人 |
DAVID L. WILSON;JOHN E. LINZ;JOHN B. KANEENE;LINDA S. MANSFIELD;ROBERT D. WALKER;THOMAS C. NEWMAN |
分类号 |
C07H21/00;C12Q1/68 |
主分类号 |
C07H21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|