发明名称 |
PROCESS FOR DNA REPLICATION |
摘要 |
A method is provided for replicating DNA, and in particular for replicating large segments of DNA. A primer is combined with a target DNA molecule to be replicated. The primer is designed to be at least partially homologous to a known site on the target DNA, and to create a D-loop when hybridized with that site. A replisome is then assembled at the D-loop, and this replisome creates a copy of the DNA, starting at the primer binding site. By utilizing two species of D-loop primers which bind to remote sites on the DNA flanking a region to be replicated, large sections of DNA can be replicated in a manner comparable to PCR. The replicated DNA can be analyzed to detect variations in the genetic sequence of the target, for linkage mapping and as a source of longer DNA molecules having a desired sequence.
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申请公布号 |
WO0046408(A1) |
申请公布日期 |
2000.08.10 |
申请号 |
WO2000US04445 |
申请日期 |
2000.02.03 |
申请人 |
SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH;MARIANS, KENNETH;JOING, LIU |
发明人 |
MARIANS, KENNETH;JOING, LIU |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68;C12N9/00 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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