发明名称 |
Methods and compositions for use in high fidelity polymerase chain reaction |
摘要 |
Methods and compositions are provided for performing high fidelity polymerase chain reactions. In the subject methods, primer extension products are produced with a low error frequency rate through the use of at least one of unequal concentrations of dNTPs or a melting point reducing agent. Also provided are kits and reagent mixtures for use in the subject methods. The subject invention finds use in a variety of applications, particularly in applications where high fidelity PCR is desired.
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申请公布号 |
US5976842(A) |
申请公布日期 |
1999.11.02 |
申请号 |
US19970960718 |
申请日期 |
1997.10.30 |
申请人 |
CLONTECH LABORATORIES, INC. |
发明人 |
WURST, HELMUT |
分类号 |
C12Q1/68;(IPC1-7):C12P19/34;C07H21/00;C07H21/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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