Method for producing viral vector

摘要

The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.

主权项

1. A method of producing a retrovirus vector, which comprises:
a step of culturing a cell producing the retrovirus vector in a culture medium containing retinoic acid, a histone deacetylase inhibitor and a substance forming a chelate as active ingredients, and a step of harvesting a supernatant containing the retrovirus vector from the culture medium, wherein the substance forming a chelate is lactobionic acid or a salt thereof.