发明名称 |
POLYNUKLEOTID-EINFANG-ASSAY UNTER VERWENDUNG VON IN-VITRO-AMPLIFIKATION |
摘要 |
An analyte polynucleotide strand having an analyte sequence is detected within a sample containing polynucleotides by contacting the analyte polynucleotide with a capture probe under hybridization conditions, where the capture probe has a first binding partner specific for a solid-phase second binding partner. The resulting duplex is then immobilized by specific binding between the binding partners, and non-bound polynucleotides are separated from the bound species. The analyte polynucleotide is optionally displaced from the solid phase, then amplified by PCR. The PCR primers each have a polynucleotide region capable of hybridizing to a region of the analyte polynucleotide, and at least one of the primers further has an additional binding partner capable of binding a solid-phase binding partner. The amplified product is then separated from the reaction mixture by specific binding between the binding partners, and the amplified product is detected. |
申请公布号 |
DE69130501(D1) |
申请公布日期 |
1998.12.24 |
申请号 |
DE1991630501 |
申请日期 |
1991.03.22 |
申请人 |
CHIRON CORP., EMERYVILLE, CALIF., US |
发明人 |
URDEA, MICHAEL, ALAMO, CA 94507, US |
分类号 |
C12N15/09;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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