| 摘要 |
PROBLEM TO BE SOLVED: To improve the take rate in the transplantation, reproduce the skin or the like, and permanently close a wound, by embedding the fibroblast derived from an epithelium tissue, in a sheet of agglutination human plasma proteins or agglutination human fibrin, and attaching squamous epithelium cells to a surface of the sheet. SOLUTION: The fibroblast exists in a condition that it is dispersed in a gel sheet made of fibrin or agglutination human plasma proteins, and a surface of the sheet is covered with the squamous epithelium cells, to obtain the transplantation piece. That is, the dermis fibroblast is suspended in a solution containing fibrinogen or the like, and is expanded in an incubator. Then thrombin and calcium are added to the same, to gglutinate fibrinogen, whereby the fibrin sheet in which the fibroblast is embedded, can be obtained. Then the low calcium serum-free medium or the like is added to the incubator, is left as it is for several hours, and the moisture is substituted for the culture medium. Then the human epidermis cell is sowed on the surface, to attach the cell. Whereby the transplantation piece of a high take property, can be obtained. |
