| 摘要 |
PROBLEM TO BE SOLVED: To efficiently obtain the subject compound useful as an intermediate for synthesizing medicines, etc., by stereo-selectively reducing N-benzyl-3- pyrrolidinone by an enzymatic reaction in two layers comprising an aqueous layer containing an enzyme and an organic solvent layer. SOLUTION: This method for producing optically active N-benzyl-3- pyrrolidinol comprises stereo-selectively reducing N-benzyl-3-pyrrolidinone by an enzymatic reaction. Therein, the enzymatic reaction is carried out in two layers comprising an aqueous layer containing an enzyme (e.g. glucose dehydrogenase) and an organic solvent layer comprising an ester, an alcohol an aromatic compound, an ether, a ketone, an aliphatic hydrocarbon or a halogenated hydrocarbon which can form the two layers together with the aqueous layer. In an embodiment, the N-benzyl-3-pyrrolidinone is reacted with glucose, nicotinamide adenine dinucleotide phosphate of coenzyme, etc. Thus,the objective optically active N-benzyl-3-pyrrolidinol useful as an intermediate for synthesizing medicines, etc., is efficiently obtained. |
