PROCESS FOR MODIFYING THE ENZYME 7 beta-(4-CARBOXYBUTANAMIDO) CEPHALOSPORINACYLASE AND PURIFYING SAID ENZYME IN A SINGLE CHROMATOGRAPHIC STEP
摘要
<p>A procedure for producing a modified 7 beta -(4-carboxybutanamide) cephalosporinase enzyme which can be purified in a single chromatographic step. The procedure for production of the enzyme involves: mutagenizing the gene which codes for the enzyme from Acinetobacter sp. ATCC 53891 by inserting a nucleotide sequence coding for six histidine residues; fusing the mutant gene with high-efficiency promoter DNA sequences; transforming Escherichia coli cells with the fusion gene construct; growing the transformed Escherichia coli cells; and recovering the enzyme by the use of supports which contain metal chelates. 7-Aminocephalosporanic acid is an important intermediate for the manufacture of a wide range of antibacterial agents of the cephalosporin family.</p>
申请公布号
EP0839914(A1)
申请公布日期
1998.05.06
申请号
EP19970919404
申请日期
1997.04.18
申请人
ANTIBIOTICOS, S.A.
发明人
GARCIA LOPEZ, JOSE LUIS;CORTES RUBIO, ESTRELLA;GUISAN SEIJAS, JOSE MANUEL;BARREDO FUENTE, JOSE LUIS;DIEZ GARCIA, BRUNO;COLLADOS DE LA VIEJA, ALFONSO;VITALLER ALBA, ALEJANDRO;SALTO MALDONADO, FRANCISCO