NUCLEOTIDE PROBES AND METHOD FOR DETERMINING HLA DQB1 TYPING

摘要

Nucleotide probe selected in particular among the following: TG CGT TAT GTG ACC AGA, GTG CGT CTT GTG ACC AGA, GT CTT GTA ACC AGA CAC AT, CGT CTT GTA ACC AGA TAC AT, CGT CTT GTG AGC AGA AGC AT, CG ACC GAG CTC GTG CGG CGT G, G TAC CGG GCA GTG ACG C, G ACG CCG CTG GGI CCG CCT G, G ACG CCG CTG GGG CCG CCT G, G GAG GGI ACC CIG GCI GAG T, G GAG GGG ACC CGG GCG GAG T, TCG GTG GAC ACC GTA TGC AGA C, GG ACG GAG CGC GTG CG, C ATC TAT AAC CGA GA, and their complementary sequences, being understood that the said probes have at least the underlined sequence, and can further contain one or two bases selected, respecting the sequence continuity, among the non-underlined bases. These probes are useful for determining a person's HLA DQ beta genotype. They have in particular the advantage of being useable at a single hybridization temperature, in particular 37 DEG C.