Volumetric cell quantification method and system

摘要

Enumeration of white blood cells (WBC) in leukocyte reduced apheresis products using a red helium neon laser to induce fluorescence in intercalating dye-labeled nucleated cells in samples loaded in precision rectangular capillaries. Apheresis samples are incubated with a surfactant which allows the intercalating dye to enter the WBC. A scanning instrument scans, identifies and enumerates the WBC in the apheresis sample. The system uses an adaptive intensity threshold to identify target fluorescent particles. Reagents are provided for lysing platelets or red blood cells in the apheresis product. A quaternary ammonium salt is added to prevent aggregation. The sample is presented to the scanning instrument in rectangular glass capillaries having a depth of 300 microns. The reagents and cartridge are provided in a kit form.