| 摘要 |
<p>The invention relates to DNA molecules, recombinant vectors and cell cultures for use in methods for expression of bile salt-stimulated lipase (BSSL) in the methylotrophic yeast Pichia pastoris. The DNA molecule comprising: (a) a region coding for a polypeptide which is human BSSL or a biologically active variant thereof; (b) joined to the 5'-end of said polypeptide coding region, a region coding for a signal peptide capable of directing secretion of said polypeptide from Pichia pastoris cells transformed with said DNA molecule; and (c) operably-linked to said coding regions defined in (a) and (b), the methanol oxidase promoter of Pichia pastoris or a functionally equivalent promoter.</p> |
