| 摘要 |
<p>An object of the present invention is to offer a method for the duplication and also for the amplification of gene wherein the base sequence is ambiguous. The constitution of the present invention is a method for duplication of gene, characterized in that, DNA which is complementary to RNA or double-stranded DNA of gene wherein the base sequence is ambiguous is dissociated into single-stranded DNA, then primer is partially bonded to a nonspecific position of the single-stranded DNA, DNA moiety having a complementarity to the single-stranded DNA is synthesized by DNA polymerase using said bonded primer as a starting point of the DNA synthesis and the above-mentioned operation is repeated twice or more whereby the nonspecific moiety of the gene is duplicated and also is a method of amplification of said gene using a PCR method thereafter. The merit of the present invention is that gene wherein the base sequence is ambiguous can be duplicated and amplified and that it can be utilized for elucidation of unknown pathogenic microorganisms, for diagnosis of said infectious disease and for development of vaccine therefor.</p> |
