| 摘要 |
PURPOSE: To obtain the subject substance capable of controlling the evolution of tissue inflammations without any danger of viral infection by developing a solution containing anα1-protease inhibitor on a cation exchange resin, adsorbing and removing contaminating impurity proteins and then recovering an unadsorbed fraction. CONSTITUTION: A fraction IV or a fraction IV-1 prepared by a Cohn ethanol separating method or its improved method is used as a plasma protein solution containing anα1 protease inhibitor (α1PI) and the resultant solution is treated by at least one means of dilution, dialysis or gel filtration chromatography, etc., to reduce the concentration of salts in the solution to <=about 0.02M and the pH of the solution is then regulated to 4.5-5.5. The regulated solution is subsequently brought into contact with a cation exchange resin having a selective affinity for contaminating impurity proteins other than theα1PI in the solution to adsorb the contaminating impurity proteins and theα1PI is separated into in an unadsorbed fraction to recover theα1PI. Thereby, the objectiveα1PI, useful for control and regulation of coagulation or complement systems, etc., control, etc., over the evolution of tissue inflammations, etc., and having high safety is obtained.
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