Ermittlungsverfahren und -gerät.

摘要

A test cell (5) for detecting a ligand in a liquid sample, which comprises an elongate casing (10) for housing a permeable material (12) and defining a liquid sample inlet (14), a reservoir volume (24), a test volume (22) interposed between the inlet (14) and reservoir volume (24), and a window (18) through the casing (10) at the test volume (22), the permeable material (12) being capable of transporting an aqueous solution disposed within the casing (10) and defining a flow path extending from the sample inlet (14) through the test volume (22) and into communication with the reservoir volume (24), there being present a first protein having a binding site specific to a first epitope on the ligand, the first protein being immobilized at a test site (18 min ), and being disposed within the test volume (22) in fluid communication with the flow path and visible through the window (18), and there being sorbent material (12) in the reservoir volume (24) for drawing liquid sample along the flow path and into contact with the test site (18 min ). A method of the detection of a ligand in a liquid sample, which comprises: (A) transporting along a flow path in a test cell a solution, including a liquid sample suspected to contain a ligand and a conjugate, into contact with a test site visible through a window in a wall of the test cell, the test site having immobilized thereon a first protein having a binding site specific to a first epitope on the ligand, and the conjugate comprising colored particles coupled to a second protein selected from proteins having a binding site specific to a second epitope on the ligand and proteins which bind with the first protein in competition with the ligand; and (B) continuing transport of the solution to progressively produce at the test site a complex comprising the ligand for a time sufficient to visually determine through the window whether a color is developed at the test site. o