NEUE CEPHALOSPORIN-C-ACYLASE.

摘要

A mutant cephalosporin C acylase ("CC acylase") of the formula (as its precursor before processing the said precursor form into alpha -subunit and beta -subunit thereof in a host cell): A<1><-><2><6><8>-X<1>-Tyr-X<2>-A<2><7><2><-><3><0><4>-X<3>-A<3><0><6>< -><7><7><3 > wherein A<1><-><2><6><8> is the same amino acid sequence as that from Thr<1> to Gly<2><6><8> of native CC acylase, A<2><7><2><-><3><0><4> is the same amino acid sequence as that from Gln<2><7><2> to Tyr<3><0><4> of native CC acylase, A<3><0><6><-><7><7><3> is the same amino acid sequence as that from Val<3><0><6> to Ala<7><7><3> of native CC acylase, X<1> is Met or other amino acid, X<2> is Ala or other amino acid and X<3> is Cys or other amino acid, providing that when X<1> is Met and X<2> is Ala, X<3> is an amino acid other than Cys. A DNA coding for the CC acylase is also provided, together with an expression vector containing the said DNA and a microorganism transformed with the said expression vector. The CC acylase is produced by culturing the said transformed microorganism in an aqueous nutrient medium.