摘要 |
A mutant CC acylase wherein at least one amino acid at the A1a49, Met164, Ser166, Met174, Glu358, Met465, Met506, or Met750 position of the animo acid sequence of the native CC acylase is replaced by a different amino acid, a DNA coding therefor, an expression vector containing the said DNA, a microorganism transformed with the said expression vector, the production of the CC acylase by culturing the said transformant, and use thereof for the production of a compound. The mutant CC acylase of the invention has desirable properties in terms of enzymatic potency, alteration of pH profile, efficiency of processing, and the like. |
申请人 |
FUJISAWA PHARMACEUTICAL CO., LTD. |
发明人 |
NIWA, MINEO;SAITO, YOSHIMASA;FUJIMURA, TAKAO;ISHII, YOSHINORI;NOGUCHI, YUJI |