发明名称 |
IN VITRO GROWTH OF NEUTROPHIL AND MEGAKARYOCYTE PRECURSORS IN SERUM-FREE MEDIA |
摘要 |
The invention provides serum-free, animal protein-free media formulations to be used in conjunction with hematopoietic growth factors for the in vitro growth of human neutrophil and megakaryocyte precursors. The medium is comprised of a base medium, corticosteroid, transferrin, insulin, cholesterol, ethanolamine, and human albumin. The invention also provides methods for preparing serum-free, animal protein-free suspensions of human hematopoietic precursor cells wherein the cellular component comprises at least about 16 % neutrophil precursors and at least about 1 % megakaryocyte precursors. Serum-free, animal protein-free suspensions of human hematopoietic cells are provided wherein the cellular component comprises at least about 30 %, preferably greater than 60 % neutrophil precursors. The neutrophil precursors are comprised of blast cells, promyelocytes, neutrophilic myelocytes, and neutrophilic metamyelocytes. Also provided are serum-free, animal protein-free cell suspensions wherein the cellular component comprises at least about 3 %, preferably greater than 8 % megakaryocyte precursors. Also provided are serum-free, animal-protein free cell suspensions wherein the cellular component comprises colony-forming units and cluster-forming units. |
申请公布号 |
WO9506112(A1) |
申请公布日期 |
1995.03.02 |
申请号 |
WO1994US09622 |
申请日期 |
1994.08.23 |
申请人 |
BAXTER INTERNATIONAL INC. |
发明人 |
SMITH, STEPHEN, L.;QIAO, XIAOYING;MACIUKAS, SUSAN, M.;BENDER, JAMES, G.;VAN EPPS, DENNIS, E.;LOUDOVARIS, MAUREEN, F. |
分类号 |
C12Q1/06;A61K35/12;A61K35/14;C12N5/078;C12N5/0787 |
主分类号 |
C12Q1/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|