WATER-INSOLUBLE POLYSACCHARIDE ORIGINATING IN MUSHROOM, ITS PRODUCTION, AND ANTITUMOR AGENT MAINLY COMPRISING THE POLYSACCHARIDE

摘要

PURPOSE:To obtain the active ingredient of Hohenbuehelia serotina at good efficiency by extracting a fraction there of insoluble in an organic solvent and aqueous solvent with an ammonium oxalate solution. CONSTITUTION:Fruit bodies of Hohenbuehelia seroting or a dried product thereof is extracted with ten- to hundred-fold as much an organic solvent, and then treated with hot water to obtain an extraction residue. This residue is optionally dried and extracted with a 0.5-5-3% ammonium oxalate solution three to ten times to obtain an extract. The supernatant liquid is separated and optionally concentrated to obtain a concentrate. To this concentrate is added about five- fold as much a highly pure solvent of a purity of 99% or higher, and the obtained precipitate is separated, washed and dried to obtain a water-insoluble polysaccharide comprising a galactomannoglucan protein complex of a protein content of 8.1-8.5% and a sugar content of 81-90% [the sugar composition is glucose: galactose: mannose=100: (11-13):(13-15)].