发明名称 |
SCREENING ASSAY FOR THE IDENTIFICATION OF IMMUNOSUPPRESSIVE DRUGS |
摘要 |
A method for identifying compounds capable of inducing immunosuppression by inhibiting the CD28 signal transduction pathway and T cell comprises exposing cultured T cells to one or more test compounds. The T cells are obtained from a T cell line which stably incorporates DNA sequence comprising in reading frame an enhancer region responsive to a CD 28-regulated nuclear binding protein and a marker gene. The cells are cultured under conditions which result in activation of both the T cell receptor and the CD28 receptor, resulting in enhanced expression of the marker gene. Test compounds which inhibit expression of the marker gene are considered as candidates for immunosuppressive drugs. |
申请公布号 |
WO9325712(A1) |
申请公布日期 |
1993.12.23 |
申请号 |
WO1993US05668 |
申请日期 |
1993.06.11 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
WEISS, ARTHUR;FRASER, JAMES |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12N5/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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