发明名称 |
HYBRID HUMAN/PORCINE FACTOR VIII |
摘要 |
A hybrid human/porcine coagulation factor VIII is produced by isolation and recombination of human and porcine factor VIII subunits, or by genetic engineering of the human and porcine factor VIII genes. Subunits of factor VIII that have been purified from human or porcine plasma are isolated, and hybrid human/porcine factor VIII is produced by mixing either porcine heavy chain subunits with human light chain subunits or by mixing human heavy chain subunits with porcine light chain subunits, thereby producing human light chain/porcine heavy chain and human heavy chain/porcine light chain hybrid molecules. These hybrid molecules are isolated by ion exchange chromatography. Alternatively, recombinant DNA methods are used to swap elements of porcine factor VIII for the corresponding elements of human factor VIII to produce hybrid human/porcine factor VIII. |
申请公布号 |
WO9320093(A1) |
申请公布日期 |
1993.10.14 |
申请号 |
WO1993US03275 |
申请日期 |
1993.04.07 |
申请人 |
EMORY UNIVERSITY |
发明人 |
LOLLAR, JOHN, S.;RUNGE, MARSCHALL, S. |
分类号 |
C12N15/09;A61K38/00;A61K38/43;A61P7/04;C07H21/04;C07K14/745;C07K14/755;C07K19/00;C12N5/10;C12N15/12;C12P21/02;C12R1/91;(IPC1-7):C07H21/00;C12N15/00;C12N15/11;A61K37/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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