RECOMBINANT ADENOVIRUSES FOR DETECTION OF SPECIFIC TRANS-ACTING GENE FUNCTION

摘要

Recombinant adenoviruses are provided for use in the detection of a trans-acting gene function in a target eukaryotic cell. The genome of the recombinant adenoviruses comprises nucleic acid capable of entering the target eukaryotic cell, said nucleic acid including a marker gene capable of being activated, directly or indirectly, in the presence of said trans-acting gene function, the activated marker gene mediating the formation of a detectable product whereby production of the detectable product is indicative of the presence of said trans-acting gene function in the target eukaryotic cell. The adenoviruses may be used in detecting various normal and abnormal states in eukaryotic cells, including virus infection.