| 摘要 |
PURPOSE:To obtain a new peptide, having a specific amino acid sequence and actions on maintenance of choline acetyl group transferase activity of cholinergic neutron and the amount of catecholamine at high levels under culture conditions, and useful for treating, etc., diseases in cerebral nervous function. CONSTITUTION:A hole of 2mm square is bored in the procephalic cortex of a rat on the 3rd day after its birth and Gelfoam(R) is embedded in the hole. After 1 week, the Gelfoam(R), together with tissues in the peripheral part, is taken out, directly frozen with dry ice and preserved at -80 deg.C. A culture medium in an amount of 10 times that of the Gelfoam(R) is cooled with ice and added to carry out homogenization. Ultracentrifuging treatment is subsequently carried out at 4 deg.C for 60min. The supernatant is collected and fractionated with ammonium sulfate of 30-60% saturation, and dissolved the resultant precipitates in a buffer solution. Gelfiltration of the prepared solution is performed to treat the active fraction with an ion exchange resin to collect an unadsorbed fraction and provide a protein capable of exhibiting neutrophic factor-like activity. The resultant protein is then hydrolyzed with trypsin and purified by high-performance liquid chromatography to afford the objective peptide having an amino acid sequence expressed by the formula. |
