| 摘要 |
PURPOSE:To attain to enhance stainability while enhancing the storage stability of a stained specimen, in staining a cell with an OG-6 stain solution and EA stain solution of a Papanicoloau's stain solution, by adding an org. acid for PH-adjustment or/and salts thereof. CONSTITUTION:In performing cytologic staining, a cell nucleus is stained with a hematoxylin stain solution at first and a keratinized epithelium is subsequently stained by using a stain solution, which is prepared by adding an org. acid for pH-adjustment or/and salts thereof to two kinds of stains, that is, Orange G and Tartrazine or a mixture obtained by adding phosphorus tungstate to said stains, as an OG-6 stain solution. As the org. acid, fatty acids, for example, acetic acid, citric acid or succinic acid are used alone or as a mixture comprising two or more of said acids and aromatic acids can be also used. By using two kinds of stains, that is, orange G and Tartrazine in the OG-6 stain solution, the staining of the keratinized epitathelium can be sharply performed or, by using either one of or both of two stains, that is, Acid Red and Fast Green FCF in addition to two kinds of stains, that is, Eosine Y and Light Green SFY as an EA stain solution, the storage stability of said solution is further improved. |
