A device and a method for fusing biological cells. The device is formed by a chamber having a tube for containing a cell suspension and a cover for sealing the tube. The chamber is provided with a lower electrode having a smooth and flat surface for contacting the cells precipitating in the cell suspension, and an upper electrode opposite to the lower electrode. The lower electrode forms the inner bottom surface of the tube. The upper electrode is inserted in the cover to enter the tube. The chamber is centrifuged to form the layers of cells on the lower electrode at the bottom of the tube. Then, a predetermined ac voltage is applied across the upper and lower electrodes to fuse the cells efficiently. Such a device and method are useful for the production of monoclonal antibodies or giant cells.
申请公布号
US5007995(A)
申请公布日期
1991.04.16
申请号
US19890350715
申请日期
1989.05.11
申请人
OLYMPUS OPTICAL CO., LTD.
发明人
TAKAHASHI, YASUO;SUZUKI, KAZUO;NIIMURA, TOSHINOBU;KANO, TOKIO