DNA encoding human serum albumin a (HSA), plasmids and hosts containing such DNA, and the production of HSA.

摘要

A process for the production of human serum albumin A (HSA) characterized by culturing host cells transformed with the plasmids containing a leader DNA sequence and a cDNA coding for mature HSA to produce and secrete mature HSA, and recovering the mature HSA. As the leader HSA sequence, a cDNA coding for the prepro sequence of HSA, a synthetic DNA coding for the prepro sequence of HSA, by the codons frequently used in a selected host, a DNA coding for a chimeric leader peptide, a DNA coding for MF alpha 1 prepro sequence, or a DNA coding for PHO5 signal peptide, may be used.