| 摘要 |
PURPOSE:To efficiently produce disaccharides having beta-glucoside bonds by reacting alpha-D-glucose-1-phosphate with monosaccharides using cellobiose phosphorylase as a catalyst. CONSTITUTION:Microbial cells of Cellvibrio.gilbus as a cellobiose phosphorylase and dispersed in low-temperature acetone at <=-10 deg.C and the resultant dispersion is stirred for about 30-2hr and filtered. The microbial cells are washed with acetone and then with ether and air-dried to prepare microbial cells treated with acetone. alpha-D-Glucose-1-phosphate is reacted with monosaccharides, such as D-glucose, are reacted in the presence of the above-mentioned enzyme. As a result, the disaccharides can be synthesized by enzymic reaction with facilitated mass production and use as a food material can be expected. |
