| 摘要 |
PURPOSE:To efficiently prevent the generation of the error of a detection value based on the variation in the dark signal of a photodiode array (FDA) or that in the brightness of a light source, by thermostatically holding FDA to stabilize the dark signal. CONSTITUTION:At first, because FDA and a diode 7 are mounted in the same thermostatic container 8, both of them are respectively held to a constant temp. of 30 deg.C. When an irradiation light path is closed by a blocking plate 61, the outputs, that is, the dark signals of 501 respective elements of FDA5 are detected and subjected to A/D conversion by an A/D converter 92 to be stored in a calculation part 93. Subsequently, when the light path is opened by the blocking plate 61, irradiated light is transmitted through a flow cell 2 to be spectrally diffracted by a diffraction lattice 4 and detected by FDA5 and a part thereof is detected by the diode 71. In this case, the outputs of 501 elements at the point of time (t) are respectively stored in the memory of the calculation part 93 and, at the same time, the intensity of irradiated light is detected by the diode 71 to be stored similarly. These data are calculated on the basis of a predetermined formula and the absorbancy of a specimen at the detection time (t) is calculated for each wavelength. |
