Integration of a gene into a chromosome and controllable excision therefrom.

摘要

Integrating a gene in a bacterial chromosome and controlling excision of the gene from the chromosome in response to an external stimulus, uses DNA comprising:
<UnorderedLists id="ula01" listStyle="none"><ListItem>a) a vector that is incapable of replicating autonomously in the host and that includes the gene lor a site for the insertion of the gene) and an attachment site corresponding to an attachment site on the host chromosome;</ListItem><ListItem>b) DNA capable of causing the integration of vector a) in the bacterial chromosome and DNA capable of causing the excision of that vector therefrom; and</ListItem><ListItem>c) DNA capable of effecting control of excision of vector a) from the bacterial chromosome in response to an externally controlled stimulus.</ListItem></UnorderedLists>