| 摘要 |
PURPOSE:To perform an analysis handily at a low cost with a high accuracy and a high reproducibility, by eluting a sample for a liquid chromatography using a mobile phase containing a reaction reagent to cool and measure the elution liquid by an excited light and/or ultraviolet rays after a heating reaction thereof. CONSTITUTION:A measuring section 4 is connected to a liquid chromatography 1, a reaction section 2 and a cooling section 3. Then, the temperatures of a column 9, a reaction tube 10 and a cooling tube 20 are kept at a specified level and a mobile phase 6 containing amino acid and boronic acid of a reaction reagent is discharged from a reaction liquid discharge tube 19 with a pump 7 via the column 9, the reaction tube 10, the cooling tube 20 and a photometer 17 to establish a steady state. Then, a sample is injected from a sample injection section 8 to be separated while sugars pass through the column 9 and the sugars are subjected to a heating reaction with a reaction reagent in the reaction tube 10 to be converted into a violet absorbing and fluorescent substance whose concentration is detected with a fluorescence photometer and/or a ultrasonic absorption photometer 17. This eliminates the passage for supply of the reaction agent to enable a highly accurate analysis free of noise and drift while ensuring a very high reproducibility. |
