| 摘要 |
The tissue culture of mammalian cells requires a serum component, such as foetal calf serum, which is expensive. It has now been found possible to replace part or all of the serum component by an extract from human blood plasma, particularly by a cold ethanol precipitation fraction obtainable between Cohn fraction II + III combined and Cohn fraction V or similar, but only if it is processed correctly. The invention provides such a process, which comprises chilling, homogenising, raising the pH to dissolve proteins and bringing the preparation to a physiologically tolerable pH and sterilising it. The invention is particularly application to culture of immortal cells, e.g. hybridomas, and includes the cultivated cells and cell products, in particular monoclonal antibodies, thus obtained.
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