| 摘要 |
1409930 Polymeric carriers for use in polypeptide synthesis CESKOSLOVENSKA AKADEMIE VED 6 Nov 1973 [6 Nov 1972] 51468/73 Headings C3H and C3P Carriers for use in polypeptide synthesis are prepared by chloromethylation (with formaldehyde and hydrogen chloride) of an acrylate or methacrylate gel copolymer containing hydrophilic functional groups and also aromatic rings. The aromatic rings may be present in a comonomer or may be introduced by an aftertreatment. In the former case, the polymer to be chloromethylated comprises units of (a) a hydrophilic monomer, e.g. 2 - hydroxyethylmethacrylate, 2-hydroxyethyl acrylate, acrylamide, methacrylamide, N-methyl methacrylamide, 2-aminoethyl acrylate, or 2-aminoethyl methacrylate; (b) a cross-linking monomer, e.g. ethylene glycol di-acrylate or di-methacrylate, tetraethylene glycol dimethacrylate, methacroylated pentaerythritol, methylene-bisacrylamide or divinyl benzene; and (c) an aromatic monomer, e.g. acrylanilide, methacrylanilide, phenyl acrylate, phenoxyethyl methacrylate or styrene. In the latter case, examples are given in which the polymer to be chloromethylated is obtained either by reacting a 2- hydroxyethyl methacrylate/ethylene glycol dimethacrylate copolymer with benzoyl chloride, phenyl isocyanate or diphenyl dichlorosilane; or by subjecting a 2-hydroxyethyl methacrylate/ ethylene glycol dimethacrylate/p-acetaminophenoxyethyl methacrylate to sequential hydrolysis, diazotization and coupling with #- naphthol. Polypeptides are synthesized on the above carriers by a sequential reaction procedure using different amino-acids which may contain protective end-groups. Examples are given in which first glycine and then alanine are built on to one of the above carriers and the resulting polypeptide chain then split off. |
