Liquid chromatography system with packed flow cell for improved fluorescence detection

摘要

In a liquid chromatography system, the effluent from the chromatographic column is passed through a detector flow cell, which is packed with a stationary phase that is adsorptive of the sample species to be detected. Detection of the sample species adsorbed at the stationary phase is effected by measuring fluorescence emitted from such species in response to electromagnetic radiation incident thereon at the flow cell. The fluorescing species, which are in equilibrium between the stationary phase and the mobile phase in the flow cell when fluorescence is being measured, can be detected at lower concentration thresholds than when the fluorescing species are in equilibrium only with the mobile phase.