NOVEL APPLICATIONS OF NICKEL NITRILOTRIACETIC ACID (NI-NTA) RESIN: HEMEPROTEIN REMOVAL, RECOVERY, AND PURIFICATION FROM BIOLOGICAL SAMPLES

摘要

The present invention relates to a method for the removal, recovery, and/or purification of hemeproteins or hemeprotein-hemeprotein ligand complexes from biological samples using Ni-NTA resin (nickel nitrilotriacetic acid). Furthermore, the present invention pertains to a method for removing hemoglobin from his-tagged fusion proteins produced in a rabbit reticulate lysate. In addition, the present invention relates to a method for the preparation of a stromal free blood substitute and methods for the removal of extracellular hemeproteins or hemeprotein-hemeprotein ligand complexes. The present invention further relates to a solid support, comprising materials and reagents described as above.