METHOD AND COMPOSITION FOR BLOOD SERUM CHOLESTEROL ANALYSIS

摘要

A totally enzymatic method for the quantitative analysis of cholesterol in a complex mixture containing free cholesterol and cholesterol esters comprising the steps of: (i) contacting a sample to be tested with a novel buffered aqueous test solution comprising: (a) a lipase having cholesterol esterase activity;(b) a protease; and(c) cholesterol oxidase; and(2) measuring the quantity of one of the products of the oxidation of cholesterol to determine the total amount of cholesterol in the sample under evaluation. Optionally, an indicator system for quantitatively detecting one or more of the cholesterol oxidation products, for example, peroxidase and a color indicator system which undergoes a color change in the presence of H2O2 and peroxidase may be included in the aqueous test solution. Quantification is preferably achieved using spectrophotometric, including fluorometric, techniques. According to a preferred embodiment the buffered aqueous test. solution includes a surfactant. A dry mixture of lyophilized enzymes which need only be dissolved in an accurate amount or water to provide an assay solution and element comprising a porous substrate into which the solution has been imbibed and dried are also described.