METHOD FOR PROGNOSIS OF THE EFFICACY OF ORAL IMMUNOTHERAPY FOR THE TREATMENT OF ALLERGY TO PROTEINS IN COW'S MILK

摘要

The present invention relates to a method for prognosis of the efficacy of oral immunotherapy for the treatment of allergy to proteins in cow's milk providing a solution to the problems stated in the state of the art since it provides a method which allows making a prognosis of the number of reactions that will be produced during oral immunotherapy (OIT) against proteins in cow's milk and/or an estimate of the treatment time required to achieve tolerance or desensitization during OIT against proteins in cow's milk and/or the need for pre-medication during OIT in a human subject.

主权项

1. A method for prognosis of the number of reactions during oral immunotherapy (OIT) against proteins in cow's milk and/or an estimate of the treatment time required to achieve tolerance or desensitization during OIT against proteins in cow's milk and/or the need for pre-medication during OIT in a human subject, comprising the following steps:
c. Isolating a biological sample from the subject; d. Determining the presence or absence of IgE antibodies in the biological sample of step a) against or with specificity for peptides identified with the following sequences:
a. At least one sequence selected from the group consisting of peptide sequences SEQ ID NO: 1-4 and/or fragments thereof selected from the list consisting of sequences SEQ ID NO: 18-32 and/or derivatives of sequences SEQ ID NO: 18-32 that are immunologically active; and/orb. At least one sequence selected from the group consisting of peptide sequences SEQ ID NO: 5-8 and/or fragments thereof selected from the list consisting of sequences SEQ ID NO: 33-45 and/or derivatives of sequences SEQ ID NO: 33-45 that are immunologically active; and/orc. At least one sequence selected from the group consisting of peptide sequences SEQ ID NO: 9-13 and/or fragments thereof selected from the list consisting of sequences SEQ ID NO: 46-63 and/or derivatives of sequences SEQ ID NO: 46-63 that are immunologically active; and/ord. At least one sequence selected from the group consisting of peptide sequences SEQ ID NO: 14-15 and/or fragments thereof selected from the list consisting of sequences SEQ ID NO: 64-69 and/or derivatives of sequences SEQ ID NO: 64-69 that are immunologically active; and/ore. At least one sequence selected from the group consisting of peptide sequences SEQ ID NO: 16-17 and/or fragments thereof selected from the list consisting of sequences SEQ ID NO: 70-75 and/or derivatives of sequences SEQ ID NO: 70-75 that are immunologically active; where the prognosis of the number of reactions during oral immunotherapy (OIT) against proteins in cow's milk and/or an estimate of the treatment time required to achieve tolerance or desensitization during OIT against proteins in cow's milk and/or the need for pre-medication during OIT is established by correlating the number of peptide sequences recognized by IgE antibodies in the samples from the subjects before starting OIT treatment (time 0) with reference values established based on those same peptide sequences which correlate the number of peptides recognized by IgE antibodies in the samples from the patients with the response after OIT treatment.