| 摘要 |
PROBLEM TO BE SOLVED: To provide an immunological reagent having anti-CEA bonding characteristics of mouse class III, anti-CEA mAb and immunogenicity of human mAb of human patient. SOLUTION: The humanized monoclonal antibody has a complementarity- determining regions of a parental murine class III, anti-CEA monoclonal antibody engrafted to the framework region of a heterologous antibody. The humanized monoclonal antibody retains the binding specificity of the parental murine monoclonal antibody, however, it is less immunogenic in a heterologous host than the monoclonal antibody. A preferred murine class III, anti-CEA monoclonal antibody is the MN-14 antibody and the preferred heterologous antibody is the one originating from human. The invention further provides DNA constructs and vectors for producing the humanized monoclonal antibodies and diagnostic and therapeutic conjugates using the same. |
