发明名称 |
Detecting cow, sheep and pig prions in a sample and transgenic animal used for same |
摘要 |
Transgenic animals are produced which animals have (1) their endogenous PrP gene ablated; and (2) have an exogenous PrP gene from a genetically diverse animal. The transgenic animal is preferably a mouse, rat or hamster with mice being particularly preferred. The exogenous PrP gene is preferably from a sheep, cow, or pig with cow PrP genes being particularly preferred. When a mouse of the invention is inoculated with a sample containing prions which generally only infects a genetically diverse species (e.g. a cow) the mouse will become ill within about 250 days or less. Methods of producing the transgenic animals are disclosed including (1) microinjecting a mouse egg (having an ablated endogenous PrP gene) with a bovine PrP gene, or (2) breeding a mouse with an ablated PrP gene with a mouse with a bovine PrP gene. Mice produced are used to test samples for the presence of prions which generally only infect cows. |
申请公布号 |
AU9295398(A) |
申请公布日期 |
1999.04.12 |
申请号 |
AU19980092953 |
申请日期 |
1998.08.28 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, |
发明人 |
STANLEY B PRUSINER;MICHAEL R SCOTT;GLENN C. TELLING |
分类号 |
A01K67/027;C07K14/47;C12N15/09;C12N15/85;G01N33/15;G01N33/50 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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