发明名称 |
PROCESS FOR THE PREPARATION OF SERUM AND PLATELET GROWTH FACTORS EXTRACT |
摘要 |
The present invention relates to a process for the preparation of platelet-serum-extract, which comprises the steps of: a) concentrating a platelet-rich-plasma prepared from whole blood by passing through a neutral support for adsorbing the platelets or by centrifuging whole blood at about 1000g to about 3000g; b) releasing the concentrated platelets of step a) by ultrasound shock; c) precipitating the concentrated plasma of step a), a platelet-poor-plasma or a serum by adding an amount of acetone solution to obtain an acetone concentration of about 68 to about 95 % at a temperature of about -15 to 5 DEG C; thereby obtaining a solid plasma or serum precipitate; d) the plasma or serum precipitate of step c) is reconstituted to a concentration of about 0.5 to 6 % of protein weight/volume and mixed to the released platelets of step b) to obtain a platelet-serum-extract of a concentration which substantially has a physiological concentration to give a maximum synergy in promoting wound healing. The present invention also relates to pharmaceutical composition for promoting wound healing, which comprises an effective concentration of a platelet-extract, a serum-extract or a platelet-serum-extract prepared according to the process of the present invention in association with a pharmaceutically acceptable carrier. |
申请公布号 |
WO9515763(A1) |
申请公布日期 |
1995.06.15 |
申请号 |
WO1994CA00601 |
申请日期 |
1994.11.01 |
申请人 |
UNIVERSITE DE MONTREAL |
发明人 |
BRAZEAU, PAUL;ABRIBAT, THIERRY;IBEA, MICHEL;BOUSHIRA, MICHELE |
分类号 |
A61K35/16 |
主分类号 |
A61K35/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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