Gene cluster for biosynthesis of griselimycin and methylgriselimycin

摘要

The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.

主权项

1. A method of producing griselimycin and/or methylgriselimycin comprising
a) providing at least one protein comprising an amino acid sequence selected from SEQ ID NOs:9, 16, 17, 19, 20, 21 and 22,
or a functionally active fragment thereof that directs synthesis of griselimycin and/or methylgriselimycin,or a functionally active variant thereof having more than 80% identity to the amino acid sequence selected from SEQ ID NO: 9, 16, 17, 19, 20, 21 and 22 that directs synthesis of griselimycin and/or methylgriselimycin and b) incubating the at least one protein or functionally active variant or fragment thereof with L-N-methylvaline, L-leucine, L-N-methylthreonine, L-proline, L-trans-4-methylproline, L-N-methylleucine and glycine to produce griselimycin and/or methylgriselimycin.