GENETICALLY ENCODED FLUORESCENT SENSORS FOR DETECTING LIGAND BIAS AND INTRACELLULAR SIGNALING THROUGH cAMP PATHWAYS

摘要

Described herein are novel fluorescent sensors for cyclic adenosine monophosphate (cAMP) that are based on single fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.

主权项

1. A cyclic adenosine monophosphate (cAMP) sensor protein comprising a first polypeptide linked to a single fluorescent protein, wherein the first polypeptide comprises a cAMP-binding domain, wherein the single fluorescent protein consists of an uninterrupted amino acid sequence, and wherein binding of cAMP to the cAMP-binding site alters the level of fluorescence from the fluorescent protein.