Compositions for cell culture and methods of using the same

摘要

Supplementation of the bioflavonoids such as epigallocatechin gallate, rutin, naringin, or genistein into mammalian cell culture media are shown to be effective in reduction of acidic species variants on recombinant antibodies. The demonstrated reduction in acidic species through the use of bioflavonoids, facilitates the manufacturing of a less heterogeneous product with potential improvements in antibody structure and function.

主权项

1. A composition comprising a chemically-defined medium (CDM) and a bioflavonoid selected from the group consisting of epigallocatechin gallate (EGCG), rutin, naringin, and genistein, wherein said bioflavonoid is present in said composition at a concentration ranging from about 0.001 g/L to about 0.2 g/L.