| 摘要 |
The present invention includes methods and recombinant DNA molecules for production of human platelet factor-4 (PF-4), modified forms of PF-4 such as MPF-4 and CPF-4, and precursor polypeptides of PF-4, MPF-4 and CPF-4. The precursor polypeptides have the formula X-PF-4, X-MPF-4, or X-CPF-4 wherein X is a cleavable peptide leader sequence. The cleavable peptide leader sequence is preferably Met-Arg or Met-Arg-Met. The precursor polypeptides can be optionally cleaved to remove the cleavable peptide leader sequence to form biologically active native compounds or can be used without cleavage in methods to inhibit angiogenesis. |
