Analyte mass spectrometry quantitation using a universal reporter

摘要

Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter.

主权项

1. A mass spectrometry (MS) analyte quantitation method comprising
preparing from a sample containing an analyte, the analyte having a known defined sequence, a proteotypic sequence being a heavy isotope labeled sequence or part thereof that fragments into a predictable ion series following MS dissociation to specifically identify and quantitate the analyte, the proteotypic sequence cleavably linked to a reporter R sequence; providing into the sample a heavy isotope labeled universal reporter U having a sequence identical to the reporter R sequence but independent from the analyte sequence; effecting cleavage; and analyzing the sample by MS parent/precursor m/z values generated using at least one of high energy or low energy to result in a response, the response used to quantitate universal reporter U and to thereby quantitate reporter R which is in equimolar concentration to the proteotypic sequence heavy isotope labeled analyte, thereby determining the quantity of analyte in the sample.