发明名称 |
Evaluation of changes in breast cells using nipple aspirate fluid |
摘要 |
This invention provides a method for studying chromosomal gains and losses in cells from nipple aspirate fluid comprising the steps of: (1) aspirating fluid from breasts; (2) placing the samples of fluid obtained in step 1 onto dishes containing conditioned medium composed of a mixture of (a) supernatant from immortalized mammary epithelial cells and (b) mammary epithelial growth medium; (3) incubating the product of step 2 in a humidified incubator; (4) replenishing the medium in the dishes prepared in step 2 at regular intervals to maintain cell growth; (5) isolating the cells from the cultures; (6) preparing DNA from these cells; (7) amplifying the DNA; (8) labeling an aliquot of the amplified DNA; and (9) evaluating the labeled DNA for evidence of chromosomal gains or losses.
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申请公布号 |
US6316189(B1) |
申请公布日期 |
2001.11.13 |
申请号 |
US19980176928 |
申请日期 |
1998.10.23 |
申请人 |
GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE |
发明人 |
HADDAD BASSEM R.;DICKSON ROBERT B.;MCCORMACK STEPHEN J. |
分类号 |
C12N5/08;C12N15/12;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12N5/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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