MONOCLONAL ANTIBODY AND DETERMINATION OF AMP DEAMINASE USING THE SAME

摘要

PURPOSE:To obtain a monoclonal antibody by immunizing an animal with an AMP deaminase purified from sheep brain, fusing the obtained spleen cell of the animal to a myeloma cell and culturing the obtained hybridoma. CONSTITUTION:AMP deaminase separated from sheep brain is purified by various column chromatography processes until the enzyme becomes a simple substance judged by electrophoresis. The purified deaminase is used as an immunogen and an animal is immunized with the enzyme. The spleen cell of the animal is collected and fused with a myeloma cell to obtain a hydridoma. The hybridoma is cultured and the objective monoclonal antibody is separated from the supernatant liquid of the cultured product or the hybridoma is transplanted into an animal body and the monoclonal antibody is separated from the ascites. The obtained monoclonal antibody can be used for the quick detection and determination of AMP deaminase in body fluid, etc.